Laboratory manual of Biochemistry and Biotechnology is a detailed manual presenting the various tests and techniques that performed in biochemistry and biotechnology laboratories. This manual is written in simple language so that every student can understand the concepts and basics of biochemistry and biotechnology in an easy manner. It would be quite easy to follow the protocols that are provided in
an easy format for various estimations. It is believed that students would surely gain hands on experience while performing the experiments in laboratories. It will broaden their knowledge and interest in science.
Features
Efforts have been made to make tables for each observation included in estimation of various metabolites
Each experiment include principle, reaction and procedure that can be followed while experimenting.
The manual has been prepared with illustrations for easy understanding.
Experiment 1: Qualitative tests for the detection of carbohydrates in given sample
Experiment 1 a: Detection of carbohydrates by molisch’s Test
Experiment 1b: Detection of reducing sugar
Experiment 1c: Distinguish between monosaccharide/disaccharide
Experiment 1d: Distinguish between ketohexose from aldohexose
Experiment 1e: To detect the presence of pentoses
Experiment 1f: To determine the presence of polysaccharide
Experiment 2: Qualitative tests for proteins and Amino acids
Experiment 2a: Protein Precipitation
Experiment 2b: Biuret Test
Experiment 2c: Xanthoproteic Test
Experiment 2d: Millon’s Test
Experiment 2e: To identify the presence of amino acids by ninhydrin test
Experiment 2f: To identify the presence of sulfur containing amino acids by amino sulfur test
Experiment 2g: To detect the presence of arginine by Sakaguchi test
Experiment 2h: To detect the presence of tryptophan by Hopkin Cole test
Experiment 3: Quantitative determination of total soluble sugars by Dubois method
Experiment 4: Quantitative determination of total proteins
Experiment 4b: Quantitative determination of total proteins by Micro-Kjeldal Method
Experiment 5: Extraction of Oils/Lipids from Oilseed using Soxhlet Method
Experiment 5a: To determine the amount of free fatty acid in given sample.
Experiment 6: To estimate amino acids by volumetric method
Experiment 7: To study the activity of enzyme (Acid Phosphatase) in germinating moongbean
Experiment 7a: Preparation of standard curve using known concentration of p-nitrophenol
Experiment 7b: To determine the activity of acid phosphatase in germinating moongbean
Experiment 7c: To determine the effect of pH on the activity of acid phosphatase in germinating moongbean
Experiment 7d: To determine the effect of temperature on the activity of acid phosphatase in germinating
moongbean
Experiment 7e: To determine the effect of substrate concentration on the activity of acid phosphatase in
germinating moongbean
Experiment 8: Demonstration of chromatographic techniques
Experiment 9: Sterilization techniques
Experiment 10: Composition of tissue culture media and preparation of MS medium
Experiment 11: To induce callus from explant
Experiment 12: Micropropagation of important crops, hardening and acclimatization of regenerated plants
Experiment 13: Isolation of genomic DNA from plant.
Experiment 14: Demonstration of Gel Electrophoresis Technique
Experiment 15: DNA Fingerprinting