Laboratory manual of Biochemistry and Biotechnology is a detailed manual presenting the various tests and techniques that performed in biochemistry and biotechnology laboratories. This manual is written in simple language so that every student can understand the concepts and basics of biochemistry and biotechnology in an easy manner. It would be quite easy to follow the protocols that are provided in
an easy format for various estimations. It is believed that students would surely gain hands on experience while performing the experiments in laboratories. It will broaden their knowledge and interest in science.

Features

 Efforts have been made to make tables for each observation included in estimation of various metabolites
 Each experiment include principle, reaction and procedure that can be followed while experimenting.
 The manual has been prepared with illustrations for easy understanding.

 Experiment 1: Qualitative tests for the detection of carbohydrates in given sample
 Experiment 1 a: Detection of carbohydrates by molisch’s Test
 Experiment 1b: Detection of reducing sugar
 Experiment 1c: Distinguish between monosaccharide/disaccharide
 Experiment 1d: Distinguish between ketohexose from aldohexose
 Experiment 1e: To detect the presence of pentoses
 Experiment 1f: To determine the presence of polysaccharide
 Experiment 2: Qualitative tests for proteins and Amino acids
 Experiment 2a: Protein Precipitation
 Experiment 2b: Biuret Test
 Experiment 2c: Xanthoproteic Test
 Experiment 2d: Millon’s Test
 Experiment 2e: To identify the presence of amino acids by ninhydrin test
 Experiment 2f: To identify the presence of sulfur containing amino acids by amino sulfur test
 Experiment 2g: To detect the presence of arginine by Sakaguchi test
 Experiment 2h: To detect the presence of tryptophan by Hopkin Cole test
 Experiment 3: Quantitative determination of total soluble sugars by Dubois method
 Experiment 4: Quantitative determination of total proteins
 Experiment 4b: Quantitative determination of total proteins by Micro-Kjeldal Method
 Experiment 5: Extraction of Oils/Lipids from Oilseed using Soxhlet Method
 Experiment 5a: To determine the amount of free fatty acid in given sample.
 Experiment 6: To estimate amino acids by volumetric method
 Experiment 7: To study the activity of enzyme (Acid Phosphatase) in germinating moongbean
 Experiment 7a: Preparation of standard curve using known concentration of p-nitrophenol
 Experiment 7b: To determine the activity of acid phosphatase in germinating moongbean
 Experiment 7c: To determine the effect of pH on the activity of acid phosphatase in germinating moongbean
 Experiment 7d: To determine the effect of temperature on the activity of acid phosphatase in germinating
moongbean
 Experiment 7e: To determine the effect of substrate concentration on the activity of acid phosphatase in
germinating moongbean
 Experiment 8: Demonstration of chromatographic techniques
 Experiment 9: Sterilization techniques
 Experiment 10: Composition of tissue culture media and preparation of MS medium
 Experiment 11: To induce callus from explant
 Experiment 12: Micropropagation of important crops, hardening and acclimatization of regenerated plants
 Experiment 13: Isolation of genomic DNA from plant.
 Experiment 14: Demonstration of Gel Electrophoresis Technique
 Experiment 15: DNA Fingerprinting