About the Authors

The field of pharmacy is constantly evolving, driven by the need for accurate and efficient methods of
analysis to ensure the safety, efficacy, and quality of pharmaceutical products. Instrumental methods
of analysis have become indispensable tools in pharmaceutical research, development, and quality
control. The book titled “Instrumental Methods of Analysis” is a comprehensive guide that serves as a
valuable resource for B. Pharmacy students, researchers, and professionals seeking to understand
and apply instrumental techniques in pharmaceutical analysis.

Unit 1: UV Visible spectroscopy and Fluorimetry
 Spectroscopic methods are vital for the identification and quantification of drugs. This chapter delves into techniques such as UV-Vis, IR, NMR, and fluorescence spectroscopy.
 Practical applications of these techniques in pharmaceutical analysis are explored, including drug characterization, purity assessment, and stability studies.

Unit 2: IR spectroscopy, Flame Photometry, Atomic absorption spectroscopy and Nepheloturbidimetry
 This unit deals with other spectroscopic techniques like IR, Atomic spectroscopy and
nepheloturbidimetry.
 Practical applications of these techniques in pharmaceutical analysis are explored, including drug characterization, purity assessment, and stability studies.

Unit 3: Introduction to Chromatography, Column chromatography, TLC, Paper Chromatography
and Electrophoresis
 This unit includes Chromatographic Techniques like Column chromatography, TLC, Paper Chromatography and Electrophoresis.
 This chapter discusses the principles of chromatography, method development, and validation in
pharmaceutical analysis.

Unit 4: Gas Chromatography and High-Performance Liquid Chromatography
 It includes Gas Chromatography and High-Performance Liquid Chromatography which are very much essential techniques in the field of pharmaceutical analysis.
 This chapter discusses the principles of HPLC and GC, method development, and validation in
pharmaceutical analysis.

Unit 5: Ion exchange Chromatography, Gel Chromatography and Affinity Chromatography
This unit explores about Ion exchange Chromatography, Gel Chromatography and Affinity
Chromatography and out lines the principle, applications in pharmaceutical research.
“Instrumental Methods of Analysis” is a comprehensive guide that equips B. Pharmacy students and
professionals with the knowledge and skills needed to apply a wide range of instrumental techniques
in pharmaceutical analysis. It is an essential resource for anyone aspiring to excel in the
pharmaceutical industry, ensuring the safety, quality, and effectiveness of pharmaceutical products.

UV Visible spectroscopy and Fluorimetry
IR spectroscopy, Flame Photometry, AAS and Nepheloturbidimetry
Chromatography- Column Chromatography, TLC, Paper Chromatography and Electrophoresis
Gas Chromatography, HPLC
Ion Exchange, Gel and Af􀀨nity Chromatography