About the Book
Abiotic stresses, such as low or high temperature, deficient or excessive water, high salinity, heavy metals, and ultraviolet radiation, are hostile to plant growth and development, leading to great crop yield penalty worldwide.Abiotic stress factors are the non-living factors influencing the metabolism, growth and development of plants tissues at that particular time when such abiotic stress affect them. This triggers a wide range of plant responses, such as altered gene expression, cellular metabolism, changes in growth rates, and crop yields.Various physio-biochemical and molecular responses are being formulated in plants in response to abiotic stress, so the plant can survive even in such extreme environmental conditions. These stress response,are orchestrated by a complex regulatory network involving upstream signalling molecules including stress hormones, reactive oxygen species, gasotransmitters, polyamines, phytochromes, and calcium, as well as downstream gene regulation factors, particularly transcription factors.The present edited book is a modest attempt to present.
Diverse physio-molecular responses in plants in reciprocation to abiotic stress.
Conceptual approaches being used to quantify the expression of the multiple stress responses in plants.
Modus operandi illustrated in understandable pattern for the benefit of researchers, teachers and students.
Introduction
ESTIMATION OF TITRATABLE ACIDITY
Estimation of
Ascorbic Acid (Vitamin-C)
Determination of Chlorophyll
Determination of Carotene (Vitamin-A)
Separation and Determination of Carotenoids
Estimation of Total Soluble Sugar
Estimation of Reducing Sugar
Determination of Total Phenols
Estimation of True Protein
by Folin Lowerys Method
Determination of Methionine
Estimation of Proline
Determination of Tryptophan
Estimation of Pectin Substances
Determination of Furfural
Determination of Stevens Cloud Stability Test
Determination of Ethanol
Determination of Acetaldehyde
Carotenoids Estimation by HPLC
Sugar Analysis by HPLC
Organic Acid Determination by HPLC
Determination of Thiabendazole in Fruit by Ultraviolet Spectrophotometer
Determination of Peroxidase Enzyme
Determination of Polyphenol Oxidase Enzyme (PPO)
Determination of Catalase Enzyme
Isolation of Plant Genomic DNA by C-Tab Method
SDS-Page Electrophoresis of Protein