About the Book

Abiotic stresses, such as low or high temperature, deficient or excessive water, high salinity, heavy metals, and ultraviolet radiation, are hostile to plant growth and development, leading to great crop yield penalty worldwide.Abiotic stress factors are the non-living factors influencing the metabolism, growth and development of plants tissues at that particular time when such abiotic stress affect them. This triggers a wide range of plant responses, such as altered gene expression, cellular metabolism, changes in growth rates, and crop yields.Various physio-biochemical and molecular responses are being formulated in plants in response to abiotic stress, so the plant can survive even in such extreme environmental conditions. These stress response,are orchestrated by a complex regulatory network involving upstream signalling molecules including stress hormones, reactive oxygen species, gasotransmitters, polyamines, phytochromes, and calcium, as well as downstream gene regulation factors, particularly transcription factors.The present edited book is a modest attempt to present.

 Diverse physio-molecular responses in plants in reciprocation to abiotic stress.
 Conceptual approaches being used to quantify the expression of the multiple stress responses in plants.
 Modus operandi illustrated in understandable pattern for the benefit of researchers, teachers and students.

 Introduction
 ESTIMATION OF TITRATABLE ACIDITY
 Estimation of
 Ascorbic Acid (Vitamin-C)
 Determination of Chlorophyll
 Determination of Carotene (Vitamin-A)
 Separation and Determination of Carotenoids
 Estimation of Total Soluble Sugar
 Estimation of Reducing Sugar
 Determination of Total Phenols
 Estimation of True Protein
 by Folin Lowery􀀳s Method
 Determination of Methionine
 Estimation of Proline
 Determination of Tryptophan
 Estimation of Pectin Substances
 Determination of Furfural
 Determination of Stevens Cloud Stability Test
 Determination of Ethanol
 Determination of Acetaldehyde
 Carotenoids Estimation by HPLC
 Sugar Analysis by HPLC
 Organic Acid Determination by HPLC
 Determination of Thiabendazole in Fruit by Ultraviolet Spectrophotometer
 Determination of Peroxidase Enzyme
 Determination of Polyphenol Oxidase Enzyme (PPO)
 Determination of Catalase Enzyme
 Isolation of Plant Genomic DNA by C-Tab Method
 SDS-Page Electrophoresis of Protein